Fig. 7.
Donor type chimerism after transplantation of Sca-1+Lin− cells: the synergistic effect of non-alloreactive T cells and purified Sca-1+Lin− cells. Different groups of sublethally (7 Gy) irradiated C3H/HeJ recipients (H2k+) were transplanted with 2 × 105 (C57BL/6) Sca-1+Lin− cells (H2b+), 10 × 106 (C3H/HeJ × C57BL/6)F1 T cells (H2k+H2b+), or both. Peripheral blood chimerism was analyzed 30 days posttransplant by flow cytometry, monitoring the engraftment of Sca-1+Lin−cells (C57BL/6) (H2b+) (A) and (C3H/HeJ × C57BL/6)F1 T cells (H2k+H2b+) (B). Data were pooled from 5 independent experiments.