Fig. 2.
Fig. 2. Genetic mapping of the jak1,jak2a, and jak2b genes of zebrafish. (a) Segregation of the 100.T7-1/100.T7-2 SSLP jak1 polymorphism in the C32xSJD cross. The C32xSJD mapping cross was typed for a polymorphic marker derived from genomic DNA associated with the jak1 gene. PCR products amplified from genomic DNA of the haploid embryos of the C32xSJD mapping panel using the 100.T7-1/100.T7-2 primer pair were analyzed for length differences by denaturing polyacrylamide gel electrophoresis and 8 representative lanes are shown. The size variants of the products were assigned to the maternal (M) or paternal (P) genome at random, and segregation in the panel was scored. (b) Segregation of the j2A.29/j2A.30 SSLP jak2a polymorphism in the C32xSJD cross. PCR products amplified from genomic DNA of the haploid embryos of the C32xSJD mapping panel using the j2A.29/j2A.30 primer pair derived from the jak2a cDNA 3′ UTR were analyzed for length differences as described above and 8 representative lanes are shown. The size variants of the products were assigned to the maternal (M) or paternal (P) genome at random, and segregation in the panel was scored. (c) Segregation of the j2B.M1/j2B.M4 SSCP jak2bpolymorphism in the C32xSJD cross. PCR products from genomic DNA of the haploid embryos of the C32xSJD mapping panel using the j2B.M1/j2B.M4 primer pair derived from the jak2b cDNA 3′ UTR were analyzed for sequence differences by nondenaturing polyacrylamide gel electrophoresis and 8 representative lanes are shown. The size variants of the products were assigned to the maternal (M) or paternal (P) genome at random, and segregation in the panel was scored. (d) Genetic map position of the jak1 gene. Analysis of the segregation of the jak1-associated marker in 72 meiotic events of the C32xSJD mapping panel using the MapMaker and mapmanager programs5960 and further manual refinement places the zebrafish jak1 locus on LG6. (e) Genetic map position of thejak2a gene. Analysis of the segregation of ajak2a-associated marker in 81 meiotic events of the C32xSJD mapping panel as described above places the zebrafish jak2alocus on LG21. (f) Genetic map position of the jak2b gene. Analysis of the segregation of a jak2b-associated marker in 48 meiotic events as described above places the zebrafish jak2blocus on LG5. (g and h) Synteny of the JAK family loci between the zebrafish, mouse, and human. A schematic diagram of the syntenic relationship between segments of the chromosomes of zebrafish (LG5, 6, and 21), human (Hsa 1, 9, and 6), and mouse (Mmu 2, 4, and 17) that contain members of the JAK gene family. The syntenic segments containing JAK2 homologs are indicated in (a) and those containing JAK1 homologs in (b). Note that genes have been illustrated in the same relative positions on syntenic chromosomes; however, in situ, local gene order may vary between chromosomes. The diagram is not to scale.

Genetic mapping of the jak1,jak2a, and jak2b genes of zebrafish. (a) Segregation of the 100.T7-1/100.T7-2 SSLP jak1 polymorphism in the C32xSJD cross. The C32xSJD mapping cross was typed for a polymorphic marker derived from genomic DNA associated with the jak1 gene. PCR products amplified from genomic DNA of the haploid embryos of the C32xSJD mapping panel using the 100.T7-1/100.T7-2 primer pair were analyzed for length differences by denaturing polyacrylamide gel electrophoresis and 8 representative lanes are shown. The size variants of the products were assigned to the maternal (M) or paternal (P) genome at random, and segregation in the panel was scored. (b) Segregation of the j2A.29/j2A.30 SSLP jak2a polymorphism in the C32xSJD cross. PCR products amplified from genomic DNA of the haploid embryos of the C32xSJD mapping panel using the j2A.29/j2A.30 primer pair derived from the jak2a cDNA 3′ UTR were analyzed for length differences as described above and 8 representative lanes are shown. The size variants of the products were assigned to the maternal (M) or paternal (P) genome at random, and segregation in the panel was scored. (c) Segregation of the j2B.M1/j2B.M4 SSCP jak2bpolymorphism in the C32xSJD cross. PCR products from genomic DNA of the haploid embryos of the C32xSJD mapping panel using the j2B.M1/j2B.M4 primer pair derived from the jak2b cDNA 3′ UTR were analyzed for sequence differences by nondenaturing polyacrylamide gel electrophoresis and 8 representative lanes are shown. The size variants of the products were assigned to the maternal (M) or paternal (P) genome at random, and segregation in the panel was scored. (d) Genetic map position of the jak1 gene. Analysis of the segregation of the jak1-associated marker in 72 meiotic events of the C32xSJD mapping panel using the MapMaker and mapmanager programs59 60 and further manual refinement places the zebrafish jak1 locus on LG6. (e) Genetic map position of thejak2a gene. Analysis of the segregation of ajak2a-associated marker in 81 meiotic events of the C32xSJD mapping panel as described above places the zebrafish jak2alocus on LG21. (f) Genetic map position of the jak2b gene. Analysis of the segregation of a jak2b-associated marker in 48 meiotic events as described above places the zebrafish jak2blocus on LG5. (g and h) Synteny of the JAK family loci between the zebrafish, mouse, and human. A schematic diagram of the syntenic relationship between segments of the chromosomes of zebrafish (LG5, 6, and 21), human (Hsa 1, 9, and 6), and mouse (Mmu 2, 4, and 17) that contain members of the JAK gene family. The syntenic segments containing JAK2 homologs are indicated in (a) and those containing JAK1 homologs in (b). Note that genes have been illustrated in the same relative positions on syntenic chromosomes; however, in situ, local gene order may vary between chromosomes. The diagram is not to scale.

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