Effect of IL-10 and LPS on the gene expression of various CIS family members, IL-8, and IL-1ra in neutrophils. PMN were preincubated with or without 100 U/mL IL-10 for 15 minutes before the addition of LPS, IFNγ, or GM-CSF for the times indicated. Total RNA was extracted and analyzed by Northern blotting. This experiment is representative of 4.