Time course of CIS3 mRNA expression in PMN treated with IL-10 and/or LPS. PMN were incubated for the indicated times with 500 U/mL IL-10 in the presence or absence of LPS, before total RNA extraction and Northern blot analysis for CIS3 mRNA expression. The extent of hybridization was quantitatively analyzed in an InstantImager (Packard Instruments) and plotted after actin normalization. This experiment is representative of 3.