T cells grown from transduced thymocytes. (A) Bivariate dot plots of flow cytometric measurement of TCR-β-PE versus EGFP and CD4-APCy versus EGFP expression of T cells grown from Nef⁻ transduced thymocytes (top) and Nef⁺transduced thymocytes (bottom). Quadrants were set to include 99% of cells stained with isotypic controls and EGFP⁻ cells in lower left quadrant. Figures indicate percentage of cells present in corresponding quadrants. (B) Proliferation of Nef⁻(filled bars) and Nef⁺ (open bars) transduced T cells shown in (A), measured by ³H-thymidine incorporation. Medium, plain culture medium; coated-CD3, well coated with CD3 monoclonal antibody; cpm, counts per minute. Columns represent average of triplicate wells and error bars indicate standard deviation.