Control tests to ascertain that CD10 is synthesized and expressed by apoptotic T cells. (A) Cells were exposed to CD95 MoAb for 18 hours, gated as indicated, and sorted. The sorted cells were analyzed for SSC and FSC or apoptosis by PI staining. (B) Cells were exposed to CD95 MoAb or medium for 48 hours and triple stained in isotonic medium with PI, Annexin-V-FITC, and CD10 MoAb-PerCP. The cells that excluded PI were gated and analyzed. (C) Cells were exposed to CD95 MoAb in the presence or absence of cycloheximide (50 μg/mL) and analyzed for CD10 expression and apoptosis by Annexin-V FITC staining.