Flow cytometric analysis used to determine the number of CD34⁺ UCB cells retrieved from the NOD/SCID mouse BM 24 hours after transplantation. Dead cells were excluded using 7-AAD. In dot plot A, living CD34-PE positive cells are gated and shown in dot plot B. CD45^dim cells possessing large forward light scatter properties are gated and shown in a CD45-FITC versus CD34-PE dot plot (C). Dot plot C clearly shows that all of the gated cells in plot B belong to a clear double positive population representing the CD34⁺ UCB cells retrieved from the NOD/SCID mouse BM.