Fig. 4.
Fig. 4. Flow cytometry dot plots from 2 patients (PNH042 and PNH 043) showing conversion of GPI-deficient T cells from a naive phenotype (CD45R0−) to a memory (CD45R0+) phenotype after stimulation in an anti-CD3 solid phase culture system. Plots A and C and E and G are unstimulated CD4+ lymphocytes and CD8+ lymphocytes, respectively. These plots clearly illustrate that the GPI-deficient (CD48−) components to be predominantly CD45R0−. After stimulation, these GPI-deficient populations convert to a memory cell phenotype with greater than 96% coexpression of CD45R0+ for CD4+ (plots B and D) and CD8+ (plots F and H) subsets in both cases studied. The residual normal components (CD48+) of CD4+ and CD8+lymphocytes although showing variable proportions of memory cells in the unstimulated controls (plots A, C, E, and G), also show maximal conversion (greater than 99%) to a memory cell phenotype in all instances (plots B, D, F, and H).

Flow cytometry dot plots from 2 patients (PNH042 and PNH 043) showing conversion of GPI-deficient T cells from a naive phenotype (CD45R0−) to a memory (CD45R0+) phenotype after stimulation in an anti-CD3 solid phase culture system. Plots A and C and E and G are unstimulated CD4+ lymphocytes and CD8+ lymphocytes, respectively. These plots clearly illustrate that the GPI-deficient (CD48−) components to be predominantly CD45R0−. After stimulation, these GPI-deficient populations convert to a memory cell phenotype with greater than 96% coexpression of CD45R0+ for CD4+ (plots B and D) and CD8+ (plots F and H) subsets in both cases studied. The residual normal components (CD48+) of CD4+ and CD8+lymphocytes although showing variable proportions of memory cells in the unstimulated controls (plots A, C, E, and G), also show maximal conversion (greater than 99%) to a memory cell phenotype in all instances (plots B, D, F, and H).

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