Analysis of TPO-induced differentiation in UT-mplwt and UT-mpl▵7. (A) Cells (0.5 × 105) were cultured in TPO (25 ng/mL) for 6 days and the cell number was counted on the indicated days. (B) Cells cultured in TPO for 6 days were cytocentrifuged on glass slides and stained with May-Grunewald-Giemsa stain. UT-mplwt cells (middle panel: original magnification × 200, arrow indicates cells shown in right panel at original magnification × 600) show an increase in cell size and cell adherence and some cells become polyploid (right panel), whereas UT-mpl▵7 cells (left panel) look identical to cells cultured in GM-CSF (not shown). (C) Surface expression of CD41a was analyzed on UT-mplwt and UT-mpl▵7 cells after 6 days in TPO or GM-CSF by flow cytometry.
