Fig. 1.
Fig. 1. Undetectable NFAT1 protein expression in unstimulated UCB T cells. (A) T cells were purified from fresh MNC of 6 UCB units (lanes 1 through 6) and from 3 adult PBMC (lanes 7 through 9). Protein extracts equivalent to 3 × 105 T cells were loaded per lane and analyzed by Western blotting with a combination of anti-NFAT1 and anti–β-actin antibodies. (B) T cells were further separated into CD45RA+ and CD45RO+ expressing cells and protein extracts analyzed as above.

Undetectable NFAT1 protein expression in unstimulated UCB T cells. (A) T cells were purified from fresh MNC of 6 UCB units (lanes 1 through 6) and from 3 adult PBMC (lanes 7 through 9). Protein extracts equivalent to 3 × 105 T cells were loaded per lane and analyzed by Western blotting with a combination of anti-NFAT1 and anti–β-actin antibodies. (B) T cells were further separated into CD45RA+ and CD45RO+ expressing cells and protein extracts analyzed as above.

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