The GC absence affects fetal liver thymic precursors. CD4/CD8/TcRβ expression in thymocytes generated in dGuo-treated murine thymic lobes after 12 days of in vitro recolonization with fetal liver cells from either 13-day-old control Sham (up) or Adx (down) rat fetuses. Histograms represent TcRβ expression in the gated DN (CD4⁻CD8⁻), DP(CD4⁺CD8⁺), and SP (CD4⁻CD8⁺ and CD4⁺CD8⁻) thymic cell populations shown in the dot plots. Results shown are a representative experiment with cell recoveries from RL-Sham of 85,000 cells/lobe and of 90,000 cells/lobe in the case of RL-Adx.