Fig. 5.
Effect of DT on the development of apoptotic nuclei in PMN maintained in culture with GM-CSF. The presence of DT (kindly provided by Dr E. Papini) in the cultures inhibited specifically the protective effect of GM-CSF on PMN apoptosis. PMN suspensions, cultured for 44 hours alone or with 25 ng/mL GM-CSF in the presence or absence of 10−8 mol/L DT, were processed for DNA content analysis by propidium iodide staining and flow cytometry analysis. Data were plotted as red fluorescence intensity versus number of nuclei with a given DNA content as determined in each experimental condition. Numbers reported indicate the percentage of hypodiploid (apoptotic) nuclei. Similar results were observed in PMN isolated from 3 independent donors.
