Ranges of sensitivities to MTX, TMQ, GW1843U89, MTA, Raltitrexed, and ZD9331 as determined by the in situ TS inhibition assay for (A) c/preB-ALL; (B) relapsed c/preB-ALL (rALL); (C) T-ALL; and (D) AML. Number of samples tested are depicted at the bottom. Median values are represented by squares, the 25th and 75th percentiles by horizontal lines. Closed squares represent the short-term exposure, open squares the continuous exposure. For ZD9331, which has not been assayed for T-ALL samples, the closed squares represent a median TSI_50,short of >25 μmol/L.