Analysis of genomic and complementary DNA from the patient with G6PD Volendam and her family. A PCR product, containing the region around 514C of the G6PD gene, was generated from genomic or cDNA obtained from blood leukocytes or cultured fibroblasts, and was analyzed by Dye Primer Cycle sequencing. Starting material: left, genomic DNA from leukocytes of the father and the mother of the patient, the patient, and her 2 daughters; right, cDNA from leukocytes of a healthy control and the patient; and cDNA from cultured fibroblasts of a healthy control and the patient. Arrows indicate mutant sequence.