Electrophoretic mobility of rG6PD B and rG6PD Volendam. rG6PD B and rG6PD Volendam were electrophoresed in native 5% polyacrylamide gel. The gels were stained for G6PD activity by PMS and MTT, as described.15 Left: Bacterial crude extract of E coli DR612 containing pMPM-A4 encoding human G6PD B (B) or G6PD Volendam (Vol). From top to bottom, arrows indicate the 70%, 95%, and 100% mobilities. Right: rG6PD B and rG6PD Volendam purified by affinity chromatography.