Integrin 6/β1 binding to laminin promotes the preferential migration of Th1 cells. (A) Mixed fluorescent-labeled Th1 (□) and Th2 (▪) cells were added to triplicate uncoated (n.c.), fibronectin- (FN), or laminin- (LM) coated transwell filters. (B) Mixed fluorescent-labeled Th1 (□) and Th2 (▪) cells were incubated with anti-integrin 6 blocking GoH3 antibody (anti-6) or an isotype-matched control (con.Ig) 15 minutes before the assay and then were added to triplicate FN- or LM-coated transwell filters.