Structural analysis of the integrated β-globin BAC transgenes. (A) Top: short-range map of BAC transgenic mouse lines. K562 DNA (lane 1), wild-type mouse ES cell DNA (lane 2), and tail DNA from F1 transgenic mice (lanes 3-11) was digested withEcoRI and a Southern analysis was performed. The blot was hybridized simultaneously with radiolabeled probes for HS-4, ɛ, γ, and β; it was then stripped and rehybridized with a probe for 3′HS-1. Bottom: EcoRI restriction map of the BAC transgene. The sizes of relevant restriction fragments are shown below the diagram of the β-globin cluster. (B) Top: Splenocytes from F1 transgenic mice (lanes 1-9) were embedded in agarose plugs. High-molecular-weight DNA was prepared, digested withKpnI, and a Southern analysis was performed. The blot was hybridized sequentially with probes for HS-4, ɛ, γ, and β. Bottom: KpnI restriction map of the BAC transgene. *KpnI derived from the pBeloBAC vector; E, EcoRI; K,KpnI.
