FCA of peripheral blood cells of a mouse mosaic forlox-▵Piga-lacZ (▵PIGA) and a normal age matched control mouse. Monoclonal antibodies against CD24, Gr−1, and CD48 were used to determine the proportion of PIGA(−) cells in individual blood cell lineages (see Materials and Methods). The left upper quadrant displays the proportion of PIGA(−) cells in the individual cell lineage. Proportion of PIGA(−) cells in the ▵PIGA mouse and values of the control mouse in parenthesis: RBCs: 10% {0%}, granulocytes: 22% {1%}, B cells 15% {0%}, T cells: 35% {0%}, CD8⁺: 31% {0%}, CD4⁺: 33% {0%}.