Fig. 3.
![Fig. 3. HA-1 gene expression in 65 HLA-A2–positive healthy blood donors, hematopoietic cell lines, and primary leukemic blasts by allele-specific RT-PCR. PCR using HA-1 allele-specific primers was performed as previously described,2 resulting in a 255-bp fragment. Results from representative samples from homozygous (HA-1H/H) (including CROFT and cell line HH [6574805; kindly provided by Dr E. Goulmy]) and heterozygous (HA-1H/R) HA-1–positive cell lines (OPM2 and KG1) or primary leukemic blasts (1, 2, and 3) as well as HA-1–negative cell lines (U266, RR [line 6574803]) are demonstrated.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/12/10.1182_blood.v94.12.4374/5/blod42446003w.jpeg?Expires=1735837000&Signature=b353mBjYjZdv3vtmeakTbOdjioB4XMnGjRsijhyENg4r8k5vps5QA1DlFjFeKwFk9XaGL5X6e2m6prBTBXv6kSXBZpryOazW9tGOTtbohoWKt~YSJpJaHjUFlM9eRS292oKXXXdBE9rqmZJPSGKnzgdVJaN~BdGfgYZn8kzdypgnwHjXkI8QMYvAxwctSo9wiU~G0zYiNtD8uVL1hugx8VFbhwh9MIuGG2DilKM-NPYPn31TyVAFQ8vB2YMlpPTAThPs2qk~jAlLT3mLPTNHdJU8kJINrq5tnlbSeYOnj~Q1BQB48T4f23UnzOZdObvOEmVp15KpDWOApO5IboiGxA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
HA-1 gene expression in 65 HLA-A2–positive healthy blood donors, hematopoietic cell lines, and primary leukemic blasts by allele-specific RT-PCR. PCR using HA-1 allele-specific primers was performed as previously described,2 resulting in a 255-bp fragment. Results from representative samples from homozygous (HA-1H/H) (including CROFT and cell line HH [6574805; kindly provided by Dr E. Goulmy]) and heterozygous (HA-1H/R) HA-1–positive cell lines (OPM2 and KG1) or primary leukemic blasts (1, 2, and 3) as well as HA-1–negative cell lines (U266, RR [line 6574803]) are demonstrated.
