SDF-1β induced calcium mobilization of B-cell subsets sorted from adult bone marrow. Sorted B cells were loaded with Indo-1AM and stimulated at the indicated time points (arrows) with the SDF-1 (100 nmol/L) or anti-IgM (2 μg/mL). The calcium mobilization is expressed as the violet/green ratio during the indicated time course. As a positive control, cells were stimulated with anti-IgM MoAb. The results are representative of 4 experiments using primary B cells from adult bone marrow. Two experiments were done on pro-B and pre-B cells from fetal bone marrow. In addition, data were confirmed by analysis of calcium mobilization of developmental stage specific B-cell lines: REH (pro-B), 697 (pre-B), Nalm-6 (pre-B) and 15A (mature).