GFP expression in cultured MKs after adenoviral transduction. CD34⁺ cells were cultured with PEG-rHuMGDF for 11 days before incubation with AdCMVhGFP (MOI of 200), viral control (MOI of 200), or vehicle control. Cells were harvested after 72 hours of viral infection and incubated with PE-GPIb antibody. FITC fluorescence (FL1) histograms were generated from the MK population for samples incubated with AdCMVhGFP (shaded), viral control (dotted line, unshaded), and vehicle control (solid line, unshaded). M1 is 1%, 2%, and 46% positivity, respectively, for vehicle control, viral control, and AdCMVhGFP. Data are representative of 2 experiments performed.