Fig. 1.
CDP binds to the −102 to −65 bp region of the gp91phox promoter. EMSA was performed as described in Materials and Methods, with the −102 to −65 bp region of the gp9phox promoter as a probe and nuclear extract isolated from the indicated cell lines. Antiserum directed against CDP, normal guinea pig serum, or 20 ng of double-stranded oligonucleotide competitor was added to the binding reactions where indicated. The E36 oligonucleotide18 is a high affinity CDP-binding site, whereas the -globin oligonucleotide20 contains a CCAAT-box motif from that gene’s promoter and serves as a heterologous competitor. Arrows indicate CDP complexes. Relative DNA/protein complex mobilities cannot be directly compared between different cell lines.
