American Society of Hematology

Fig. 4.

$Fig. 4. ICSBP and IRF-4/Pip form a complex in B cells and macrophages. (A) IRF-4/Pip is precipitated out of mouse B-lymphocyte extracts with an antibody against ICSBP. (A) Shows a mobility shift assay incubating the ISG15 ISRE oligonucleotide with wild-type mouse B-lymphocyte extracts, either mock-depleted (lanes 1 to 4) or depleted for the ICSBP protein (lanes 5 to 8). In lanes 2 and 6, the extracts are incubated with antibody against ICSBP, lanes 3 and 7 are with anti-IRF-4/Pip, and lanes 4 and 8 are with anti-IRF–2. (B and C) Evidence for ICSBP and IRF-4/Pip complex by coimmunoprecipitation in B-cell line, A20.2j (B) and in primary macrophages (C). The IRF-4/Pip protein was precipitated with an antibody against ICSBP and vice versa as described in Materials and Methods. Fluorographs of the immunoprecipitated fractions (P) and the supernatants (S) are shown.$

ICSBP and IRF-4/Pip form a complex in B cells and macrophages. (A) IRF-4/Pip is precipitated out of mouse B-lymphocyte extracts with an antibody against ICSBP. (A) Shows a mobility shift assay incubating the ISG15 ISRE oligonucleotide with wild-type mouse B-lymphocyte extracts, either mock-depleted (lanes 1 to 4) or depleted for the ICSBP protein (lanes 5 to 8). In lanes 2 and 6, the extracts are incubated with antibody against ICSBP, lanes 3 and 7 are with anti-IRF-4/Pip, and lanes 4 and 8 are with anti-IRF–2. (B and C) Evidence for ICSBP and IRF-4/Pip complex by coimmunoprecipitation in B-cell line, A20.2j (B) and in primary macrophages (C). The IRF-4/Pip protein was precipitated with an antibody against ICSBP and vice versa as described in Materials and Methods. Fluorographs of the immunoprecipitated fractions (P) and the supernatants (S) are shown.

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