Fig. 2.
![Fig. 2. Time courses of [32P]PtdIns, [32P]PtdIns(4)P, [32P]PtdIns(4,5)P2, and [32P]PtdOH production and effects of released ADP in TRAP-stimulated platelets. Time courses of [32P]-labeling of PtdIns, PtdIns(4)P, PtdIns(4,5)P2, and PtdOH in [32P]-labeled platelets activated by 40 μmol/L TRAP in the absence (•) or the presence (○) of 1 U/mL apyrase. Radioactivity incorporated into various phospholipids was quantified using HPLC as in Fig 1. Results are representative of 3 experiments with similar results.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/12/10.1182_blood.v94.12.4156/5/blod42429002x.jpeg?Expires=1735530798&Signature=YhYfiMsJNhXpXTeDdOMWeuGDyUOmd6lSzI87DQ1hg-YBGMtvW3oTgP12wTWQ5kKa7s1s~0aBwfFKrh64IwJlo7vjsQQkp9~08VF7AhNb9z7GbWW9p-jD2P1sTpn8HsqG~t-tra1uzzx5SX7c5KLCXfUizsWPmkGJmV9xuD84KJ07uL2yNvqa0hbo7GYtBzYjQkh7Bh1ckgSUwSnf0cFlx71qIS8RpL5ToHnzHdWbVvDIvaH-i1zWaCTBSLDOJ7PO4aiZAaGDJ7NIx5HftflYSos50Ka4yzKtI9DZuU8-jvTL-fMt64qRbzHaqPswXpFSbPwhsDtkOra5ChlF0HXy3Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Time courses of [32P]PtdIns, [32P]PtdIns(4)P, [32P]PtdIns(4,5)P2, and [32P]PtdOH production and effects of released ADP in TRAP-stimulated platelets. Time courses of [32P]-labeling of PtdIns, PtdIns(4)P, PtdIns(4,5)P2, and PtdOH in [32P]-labeled platelets activated by 40 μmol/L TRAP in the absence (•) or the presence (○) of 1 U/mL apyrase. Radioactivity incorporated into various phospholipids was quantified using HPLC as in Fig 1. Results are representative of 3 experiments with similar results.
