Transiently expressed LMP-1 down-regulates CD99 promoter-driven luciferase activity in 293T.

(A) Schematic presentation of the CD99 promoter-driven luciferase construct pCD99(−1650/+130)luc. Closed bars denote putative Sp1-binding sequences located at −287, −255, −164, −107, −61, −18, +11, and +61 relative to the transcription start site indicated as an arrow head. (B) Effect of LMP-1 expression on CD99 promoter activity. The mean values for 2 experiments are shown. The histogram shows the relative lucifersase activity normalized by the internal control plasmid, pBiP670CAT.