Inhibition of PAF-induced changes in L-selectin and CD18 expression on leukocytes. Whole blood aliquots were incubated with LXA4 analogs for 30 minutes and then challenged with 1 μmol/L PAF for 30 minutes at 37°C. Adhesion molecule expression is presented as the percentage of control (unchallenged cells). Mean fluorescence intensity for L-selectin: PMNL, control, 103 ± 9; PAF, 43 ± 5; monocytes, control, 30 ± 3; PAF, 20 ± 2; lymphocytes, control, 53 ± 3; PAF, 50 ± 2, n = 5, all P < .05. Mean fluorescence intensity for CD18: PMNL, control, 48 ± 3; PAF, 61 ± 6; P < .05; monocytes, control, 107 ± 12; PAF, 123 ± 13;P < .05; lymphocytes, control, 19 ± 1; PAF, 21 ± 1. The results are the mean ± SEM of 4 to 5 experiments with different donor cell preparations. *P < .05 vPAF-stimulated cells.
