Effect of 15-R/S-methyl-LXA4 on the expression of LFA-1 epitope expressed by the β-propeller domain of LFA-1 on neutrophils. Mean fluorescence of MoAb G-25.2 on PMNL in whole blood challenged with PAF (1 μmol/L; A) or IL-8 (10 nmol/L; B) for 30 minutes at 37°C in the presence of 15-R/S-methyl-LXA4(5 μmol/L). The dotted line represents MoAb G-25.2 binding at 4°C. Shown is a representative of 3 experiments. (C) The expression of MoAb G-25.2 on PMNL in whole blood challenged with Mg2+ (1 mmol/L) and EGTA (2 mmol/L) for 30 minutes at 4°C (dotted line) and at 37°C in the absence or presence of 15-R/S-methyl-LXA4 (5 μmol/L). Shown is a representative of 3 experiments. (D) Mean fluorescence of MoAb G-25.2 on neutrophils challenged with Mg2+ and EGTA in the presence of 15-R/S-methyl-LXA4. Values are the means ± SEM of 3 experiments.
