Patterns of reactivity of IgG purified from plasma of patients and healthy blood donors with self-antigens.

IgG purified from plasma of patients with WAIHA and healthy blood donors was interacted at 200 μg/mL with self-antigens, as described in Materials and Methods. The figure depicts the mean densitometric profiles of reactivity of purified IgG (i.e., the arithmetic mean of the recorded intensities constituting the reactivity profile of each individual) of 20 patients with WAIHA (black area) and 20 healthy blood donors (gray area) with antigens in soluble extracts of normal RBCs (A) and normal stomach (B). White areas depict the pattern observed in the presence of secondary anti-Fcγ antibody alone. Optical densities and migration distances are expressed as arbitrary units (AU). Migration distances of 200, 600, and 1000 AU correspond to molecular weights of 200, 65, and 20 kDa, respectively.