STAT activation in resting PBMCs exposed in vitro to HIV-1. EMSA using WCEs (8 μg) obtained from resting PBMCs, NA-PBMCs, and M▹ exposed for 7 days to HIV-1_IIIB/LAI and HIV-1_BaL strains (0.1 moi) and a mixture of PRE and UCR radiolabeled oligonucleotides. After a single round of purification, CD4⁺-depleted PBMCs of patients no. 15 and 16 contained 3% and 1% CD4⁺ cells, respectively, whereas CD8⁺-depleted PBMCs contained 0.3% and 1% CD8⁺ cells, respectively.