Fig. 3.
![Fig. 3. The effect of IFN-γ neutralization in SC cultures. / SC (0.4 × 106 cells/well) were stimulated with Con A (1 μg/mL) or anti-CD3 (2 μg/mL) plus IL-2 (50 units/mL) and cultured for 72 hours with or without antimurine IFN-γ neutralizing mAb (10 μg/mL) added at culture initiation. [3H]TdR incorporation (A) and nitrite production (B) were assessed at 72 hours after the starting point. Data are the mean ±SD of triplicate cultures of pooled cells from PBS- or CTX-injected mice.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/1/10.1182_blood.v95.1.212/5/bloo00127003x.jpeg?Expires=1735517650&Signature=QB2tac~ezva3Mt7tGa~4d618zoEEMM~a3ypidMDYpuLMOPVxUHjrpHGwI6enj8nTQoYheCOuBjnEYajdUXmFz-DBpwwhuaNIcAH8MI5XSlSCrIwc8qNdqXSNmaL~OZpozXkLZwVaFDgFVDlPvwSD-2woWU9CElnbOHpY~FwmHqzohvsfKB8UqqtGyDwNnYii5aaq~esxDmZKgu8dOYn0gbeOOR8DxZNq5F4lSGFUN2aJko0~gXJrkuTBNtcAmkjD9bw7TePBolS1VXiRiwhQRGnkSS2fLUb~u-Gc7mnw8O0IG1VjMdh68A7zTN-oMCaQD4BbUrxG~h4dBjahZf-uzw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The effect of IFN-γ neutralization in SC cultures.
SC (0.4 × 106 cells/well) were stimulated with Con A (1 μg/mL) or anti-CD3 (2 μg/mL) plus IL-2 (50 units/mL) and cultured for 72 hours with or without antimurine IFN-γ neutralizing mAb (10 μg/mL) added at culture initiation. [3H]TdR incorporation (A) and nitrite production (B) were assessed at 72 hours after the starting point. Data are the mean ±SD of triplicate cultures of pooled cells from PBS- or CTX-injected mice.
