Lack of cytotoxicity of TAK-1 to the HLA-A24 mutant cell line KU812.
(A) The sequence of the HLA-A24 gene of the myeloid leukemia cell line KU812 was determined. The inserted 23-base pair repeated nucleotides are shown by underlined italics, and the insertion point is marked with an arrow. (B) The cell surface expression levels of HLA-A24 and HLA class I on the myeloid leukemia cell lines KU812 and TK91 were measured by flow cytometry. The cells were stained with an FITC-conjugated mouse anti-HLA-A24 MoAb, FITC-conjugated mouse anti-HLA class I MoAb (shaded histograms), or FITC-conjugated mouse IgG (open histograms). (C) The cytotoxicity of TAK-1 to KU812 and TK91 cells was determined by 51Cr release assays for 4 hours at an E:T ratio of 10:1 in the presence or absence of the WT1 peptide. The cytotoxicity of TAK-1 to TK91 cells preincubated with an anti-HLA class I MoAb or an anti-HLA-DR MoAb was also examined.
