Expression of ESL-1 and FucT-VII by CD34⁺cells. RT-PCR analysis was performed to examine the capacity of CD34⁺ cells to express ESL-1 and FucT-VII. (A) The expected product of 450 bp for ESL-1 could be detected in BMEC (lane 1, EC), HL60 (lane 3, positive control), PB CD34 cells (lane 4, PB), BM CD34 cells (lane 5, BM), FL CD34 cells (lane 6, FL), and CB CD34 cells (lane 7, CB). RT (lane 2) is the PCR reaction without addition of RT. (B) The expected product of 497 bp for FucT-VII could be detected in HL60 (lane 3, positive control) and in PB- (lane 4), BM- (lane 5), FL- (lane 6), CB-derived CD34(+) cells (lane 7). FucT-VII expression is only limited to hematopoietic cells, and BMEC (EC, lane 1) did not express FucT-VII. RT control (lane 2) is the PCR reaction without addition of RT.