Expression of ephrin-A4 mRNA in different human tissues and cell types.

Upper panels show ephrin-A4 hybridization; lower panels, β-actin hybridization. (A) Expression in different hematopoietic tissues: (1) spleen, (2) lymph node, (3) thymus, (4) peripheral blood leukocytes, (5) bone marrow, and (6) fetal liver. (B) Expression in freshly isolated, cultured B and T lymphocytes: (1) peripheral blood B cells, (2) 24-hour TPA-stimulated blood B cells, (3) 24-hour anti-μ–stimulated blood B cells, (4) peripheral blood CD4+ T cells, (5) 24-hour TPA-stimulated blood CD4+ T cells, (6) 24-hour anti-CD3–stimulated blood CD4+ T cells, (7) peripheral blood CD8+ T cells, (8) 24-hour anti-CD3–stimulated blood CD8+ T cells, (9) tonsil B cells, and (10) tonsil T cells. (C) Expression in hematopoietic cell lines: (1) Tom-1, (2) BV173, (3) Reh, (4) Nalm-6, (5) Daudi, (6) Bjab, (7) U266, (8) U698, (9) JM, (10) Jurkat, (11) HPB ALL, (12) JY, (13) KG1-A, 914) HL60, (15) U937, and (16) K562.