Semiquantitative PCR analysis of the expression of ephrin-A4 (m) and ephrin-A4 (s) mRNA in freshly isolated and stimulated B and T lymphocytes.

(1) water control, (2) Reh pro-B cells, (3) tonsil T lymphocytes, (4) 24-hour anti-CD3– stimulated CD8+ blood T lymphocytes, (5) 24-hour anti-CD3–stimulated CD4+ blood T lymphocytes, (6) tonsil B lymphocytes, and (7) 24-hour anti-μ–stimulated blood B cells. The upper panel shows ephrin-A4 (s) specific PCR (28 cycles); the middle panel, ephrin-A4 (m) specific PCR (30 cycles); and the lower panel, β-actin specific PCR (24 cycles).