Immunofluorescent analysis of ephrin-A4 binding tonsil adherent cells.

Tonsil adherent cells were stained with ephrin-A4-Fc (A) or ephrin-CD19short-Fc (D) chimeric proteins in combination with a panel of fluorochrome-labeled monoclonal antibodies. (B, C, E, F) Histograms represent the staining patterns of ephrin-A4 binding cells gated in the upper left panel (A): shaded histograms represent ephrin-A4 binding cells costained with relevant FITC control, and open histograms represent costaining of ephrin-A4 binding cells with FITC-labeled antibodies directed to indicated surface markers.