The effects of IL-12 and IL-4 on proliferation and cytokine production of Tc1 and Tc0/2 CD8 T-cell clones.
Cloned cells at 1 × 105/well were incubated with PMA (10 ng/mL) and ionomycin (400 ng/mL) in the presence of IL-12 or IL-4 (0 to 10 000 U/mL). All experiments were performed in triplicate. On day 4, the cells were washed and restimulated with PMA and ionomycin in the absence of cytokines. At 24 hours, supernatants were collected and the cells were incubated in the presence of3H-thymidine (0.5 μCi/well) for 6 hours and then harvested. Mean cpm values for 8 Tc1 and 8 Tc0/2 clones are shown. Levels of IFN-γ, IL-4, IL-5, and IL-10 in the supernatants were measured by ELISA (as described in Materials and Methods). All results are expressed as mean ± SEM for 8 clones under control conditions (filled bars) and at 10 000 U/mL IL-4 or IL-12 (shaded bars).
