Transient expression of wild type and mutant recombinant protein S in COS 1 cells.

(A) Western blot analysis of 10 μL of conditioned media was performed according to Materials and Methods section. (B) ELISA determination of the concentrations of the different mutants. Bars represent the mean ± SD of 16 different protein S determinations corresponding to duplicates from 8 transfection experiments per mutant. An exception is P626L, for which 4 transfections were performed. A value of 100% was arbitrarily given to the mean value of the wild type expression. Comparison between the mutant and the wild type expression levels was performed using an unpaired t test. OnlyP-values considered significant (≤ .05) are shown.