Downmodulation of NF-κB/Rel nuclear complexes by a κB decoy oligodeoxynucleotide in 32D cells. 32D cells (2 × 10⁵/mL) were incubated in the presence or absence of 7.5 μmol/L κB decoy (5′-CCTTGAAGGGATTTCCCTCC-3′) or scrambled (5′-CCTTGTACCATTGTTAGCC-3′) oligodeoxynucleotide for 16 hours. Nuclear extracts were then obtained, incubated with a³²P-labeled κB oligonucleotide, and analyzed by EMSA.