Effect of the κB decoy oligonucleotide on the NF-κB/Rel nuclear complexes in CD34⁺ cells. CD34⁺ cells (purity, >96%, ≤99%; 7.5 × 10⁵/mL) were incubated in 10% FCS-RPMI, with or without amifostine (8 μg/mL), decoy, or scrambled oligo (5 μmol/L) for 16 hours. The cells were then analyzed in immunofluorescence with an antihuman NF-κB/p65 rabbit polyclonal serum and FITC-conjugated goat antirabbit Ig antibodies.