Influence of IL-12 or TNF- on LPS-stimulated IFN-γ production in the presence or absence of G-CSF.

IL-12 (10 ng/mL) or TNF-α (10 ng/mL) or a combination of both was added to 20% whole blood in RPMI 1640. A set of samples was additionally stimulated with 10 μg/mL LPS ± 100 ng/mL G-CSF. After 24 hours of incubation at 37°C, released cytokines were measured in the cell-free supernatants by ELISA. Data represent means ± SEM of 8 different donors. ⁺⁺P < .01 versus control, *P < .05, **P < .01 versus respective LPS samples.