Phenotype and function of CD34⁺ cell subset progeny.

(A) Flow cytometric analysis of various cultures of hematopoietic progenitor cell subsets. CD34⁺Lin⁻ CD10⁺ cells in FKGm17 (left panel), or CD34⁺ Lin⁻ CD10⁻cells in FKGm17 (middle panel), or CD34⁺Lin⁻ CD10⁻ cells in FKGmT4 (right panel) were analyzed 2 weeks after the start of culture. The top row shows background values and the middle row shows the correlated expression of FITC-CD14 and PE-CD1a markers. Numbers indicate the percentage of cells in the respective regions. The bottom row shows forward scatter (FSC) and side scatter (SSC) characteristics of the cells. (B) Two representative and distinct experiments show the stimulation of purified allogeneic T cells in MLR with irradiated progenies of CD34⁺ Lin⁻ CD10⁺cells (closed circle) or of CD34⁺ Lin⁻CD10⁻cells (open square) in MLR. Proliferation (left panel) was measured by ³H-thymidine incorporation and IL-2 secretion in the medium (right panel) by ELISA.