Effects of Ik7 on DC differentiation.

Representative flow cytometric analyses of cultures of CD34⁺ EGFP⁺ cells infected with control virus (left panels) or of CD34⁺ EGFP⁺ cells infected with Ik7 virus (middle panels) and of CD34⁺EGFP⁻ control cells obtained from cultures infected with Ik7-containing virus (right panels). Progenitor cells were obtained as indicated in Figure 4 and cultured with different cytokines. (A) Dot plots show the correlated expression of the CD1a marker and EGFP correspond to 1 experiment where cells were cultured for 7 days with FKGmT4 or FKGm17. (B) A separate experiment in which cells were stimulated with FKGm17 for 11 days and analyzed for the correlated expression of CD83, CD14, EGFP, FSC, and SSC parameters. In all dot plots, the analysis is carried on live cells excluding propidium iodide. Percentages of cells comprised within defined regions are indicated on the plots.