Western blot analysis of fibrinogen in media (A and D) or in CHO cell lysates (B, C, E, and F) that were transfected with mutant or normal fibrinogen γ-chain plasmid. The samples were subjected to 8% SDS-PAGE under nonreduced conditions (A, B, and C) or 10% SDS-PAGE under reduced conditions (D, E, and F). After transfer to nitrocellulose membrane, blots were developed with a rabbit antihuman fibrinogen antibody (A, B, D, and E) or a rabbit antihuman fibrinogen γ-chain antibody (C and F) that reacted to not only human fibrinogen γ-chain, but also to Bβ-chain. The samples in each figure were (1) purified fibrinogen from normal control plasma, (2) ABβ CHO cells, (3) γ153C-7, (4) γ153C-16, (5) γ153R-24, (6) γ153R-32, (7) γ153A-7, and (8) γ153A-11.