Expression of LFA-1 and MMP-9 by 374 lymphoma cell line.

(A) Expression of LFA-1 on 164T2 (left histogram) and 374 cells (right histogram). Expression of LFA-1 was determined using biotinylated anti-LFA-1 antibodies plus SA-PE (gray lines). Controls included cells incubated with SA-PE alone (black lines) and autofluorescence control, represented by the population filled in gray. The T-cell origin of 374 cells was ascertained by the expression of the CD3ε and TcRαβ-specific mAbs (data not shown). (B) Secretion of high levels of MMP-9 by 164T2 and 374 T lymphoma cells. Secretion of MMP-9 by resting or PMA-stimulated 164T2 and 374 lymphoma cells was measured by gelatin-zymography. Results are representative of at least 3 independent experiments.