Quantitative analysis of lung eosinophils in IL-1 R type 1-deficient mice.

The number of eosinophils in cryosections of lung tissue was enumerated before and after allergen challenge and after diluent challenge in wild-type and IL-1 R type 1-deficient mice using a method to detect the presence of eosinophil peroxidase. Results are expressed as the number of lung eosinophils (expressed as eosinophils/hpf lung tissue) (n = 3 experiments). There was a significant inhibition of lung eosinophilia in IL-1 R type 1-deficient mice after allergen challenge compared with wild-type mice after allergen challenge (P = .01).