Comparisons of eosinophil rolling and firm adhesion in IL-1 R type 1-deficient and control wild-type mice using intravital microscopy.

Ragweed-sensitized mice (IL-1 R type 1-deficient or control wild-type mice) were challenged with intraperitoneal injections of ragweed. Twenty-four hours later fluorescence-labeled mouse eosinophils were infused intravenously into mouse tail veins. Mouse mesentery was visualized by intravital microscopy, and intravascular eosinophil rolling and adhesion to endothelium were recorded on videotape. The number of eosinophils rolling on endothelium in ragweed-challenged IL-1 R type 1-deficient mice was significantly less than that observed in ragweed-challenged control wild-type mice (P = .05) (panel A). Similarly, the number of eosinophils adherent to endothelium in ragweed-challenged IL-1 R type 1-deficient mice was significantly less than that observed in ragweed-challenged control wild-type mice (P = .002) (panel B). Six mice were studied in each experiment (n = 2 experiments), and 3 to 6 blood vessels were analyzed per mouse.