Inhibition of Arg2150His fVIII activity by LE2E9.

Equal volumes of buffer (open bars) or LE2E9 (10 μg/mL, solid bars) and of plasma were mixed and incubated for 1 hour at 37°C. The residual fVIII activity was measured in a fVIII chromogenic assay. Plasma samples were: pool of normal plasma (Nl), LM plasma (LM), LM plasma supplemented with pdfVIII (LM + pdfVIII), and plasma of a severe hemophilia A patient supplemented with pdfVIII (He + pdfVIII). The mean residual fVIII activities with standard deviations of 3 independent experiments are shown.