Fig. 3.
![Fig. 3. Immunoprecipitation of wild-type and mutated rfVIII light chain. / Recombinant fVIII fragments labeled with [35S]methionine and expressed in reticulocyte lysates were incubated for 2 hours at 4°C with LE2E9 (LE) bound to Protein A Sepharose. After washing, bound material was eluted by SDS-buffer and analyzed by SDS-PAGE, followed by autoradiography. Controls included a human monoclonal antibody, BO2C11 (BO), directed toward the fVIII C2 domain,19 a rabbit polyclonal IgG antibody (aA3), directed toward amino acid residues 1797-1815 of the fVIII A3 domain and normal donor polyclonal IgG antibodies (Wi). The experiments were repeated 3 times with similar results.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/1/10.1182_blood.v95.1.156/5/bloo00150003w.jpeg?Expires=1736047522&Signature=J5Xh4R83vYH4924vDuuAfsGZjBjMc301zG42fLIjyKDproEg4iD4V9HE6KaSHQUdDe1z7fhvrhC8-uWgV2s3etnXChBvkd2sHSoz8I4BtO0D-ERU28GWoO~Di7tpaoWfxyzsCVL3PgPSatvB6kQ31MC7DHkUWh3hBGyvfngBcYssk2iyaBHiMmIk5Nlcru8hdLocsjQ~qrqWCnOEZaq8xxPDAL1EeWBpmiVKceJiJQTjFfEhCBwwV-Bx32GTmtaBz9R2ov8wCrZt6k4NyxwE-p7TY~OSVNGKBsP~PNfROXuMoGd0eLn4WtwR7HMeiCLpA3yOqAtauJuOn-npMyYfVg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunoprecipitation of wild-type and mutated rfVIII light chain.
Recombinant fVIII fragments labeled with [35S]methionine and expressed in reticulocyte lysates were incubated for 2 hours at 4°C with LE2E9 (LE) bound to Protein A Sepharose. After washing, bound material was eluted by SDS-buffer and analyzed by SDS-PAGE, followed by autoradiography. Controls included a human monoclonal antibody, BO2C11 (BO), directed toward the fVIII C2 domain,19 a rabbit polyclonal IgG antibody (aA3), directed toward amino acid residues 1797-1815 of the fVIII A3 domain and normal donor polyclonal IgG antibodies (Wi). The experiments were repeated 3 times with similar results.
