Fig. 5.
![Fig. 5. Binding of LE2E9 to native or Arg2150His C1 and C1C2 domains. / Native and Arg2150His recombinant fVIII C1 domain (A) or C1C2 domains (B) labeled with [35S]methionine and expressed in reticulocyte lysates were incubated for 2 hours at 4°C with LE2E9 (LE) bound to Protein A Sepharose. After washing, bound material was eluted by SDS-buffer and analyzed by SDS-PAGE, followed by autoradiography. Controls included the human monoclonal antibody, BO2C11 (BO), and normal donor polyclonal IgG antibodies (Wi). The experiments were repeated 3 times with similar results.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/1/10.1182_blood.v95.1.156/5/bloo00150005w.jpeg?Expires=1735814296&Signature=ZemiNWvgcE8pUxFroUOqQWM4q0hY6UAfxn7pWYG9ih7-K~BxWZcbzRYajY4-UWVDZhmDt-nodiZh4JRKIsLaNbSg6Ldsw8H5julEJuKdfwYO0UKGPWpoRmjVJSH7rNJql1t~ruerpR9uNEpL2yl82E87yUcLV4HZWkl16N51fXl19yiZK45GrknKTi3gAu6M07unsVcNcW2C-twyuXMc5L3HJYvB~SU9GDlf30gM7LEoKTF2A71lI5qsOc1k9i3b6eH-ntfgMcrLI2wzVGTx2ZhQ66dlX5UUauClpFmuMrkS-TOBcgrBtsDHtQgd~Vkqae4gsC830HbdRrLWORbO3g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Binding of LE2E9 to native or Arg2150His C1 and C1C2 domains.
Native and Arg2150His recombinant fVIII C1 domain (A) or C1C2 domains (B) labeled with [35S]methionine and expressed in reticulocyte lysates were incubated for 2 hours at 4°C with LE2E9 (LE) bound to Protein A Sepharose. After washing, bound material was eluted by SDS-buffer and analyzed by SDS-PAGE, followed by autoradiography. Controls included the human monoclonal antibody, BO2C11 (BO), and normal donor polyclonal IgG antibodies (Wi). The experiments were repeated 3 times with similar results.
