Inhibition of LE2E9 binding to fVIII by LE IgG.

Biotin-labeled LE2E9 at 0.5 μg/ml was mixed with different concentrations of LE2E9 (▪), LE IgG (■), or control polyclonal IgG (○) before addition to fVIII-coated plates. The plates were then incubated for 2 hours at RT and the binding of biotin-labeled LE2E9 was detected by addition of HRP-labeled avidine. The mean and standard deviation of duplicates are indicated. Controls in the absence of fVIII gave OD₄₉₀ lower than 0.05. The experiments were repeated 3 times with similar results.